The sequence and context of the 5′ splice site govern the nuclear stability of polyoma virus late RNAs (original) (raw)

Journal Article

,

Department of Microbiology, University of Connecticut Health Center

Farmington, CT 06030, USA

• Prescnt address: Epidemiology Program, Department of Public Health. Hartford, CT 06106, USA

Search for other works by this author on:

,

Department of Microbiology, University of Connecticut Health Center

Farmington, CT 06030, USA

Search for other works by this author on:

Department of Microbiology, University of Connecticut Health Center

Farmington, CT 06030, USA

* To whom correspondence should be addressed

Search for other works by this author on:

• Prescnt address: Epidemiology Program, Department of Public Health. Hartford, CT 06106, USA

Author Notes

Published:

01 January 1995

Received:

11 September 1995

Accepted:

26 October 1995

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Nancy L. Barrett, Xiaotong Li, Gorden G. Carmichael, The sequence and context of the 5′ splice site govern the nuclear stability of polyoma virus late RNAs, Nucleic Acids Research, Volume 23, Issue 23, 11 December 1995, Pages 4812–4817, https://doi.org/10.1093/nar/23.23.4812
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

We have examined the influence of splicing signals on the stability of polyoma virus late RNAs in the nucleus. Late primary transcripts contain a single 5′ splice site and three alternative 3′ splice sites. In earlier work we showed that the presence of introns was not required for late RNA accumulation, however, the 5′ splice site was essential, as removal of only the 5′ splice site was sufficient to destabilize late RNAs up to 100-fold when compared with early RNAs. A complementary clone which retained the 5′ splice site but which carried small deletions of all late region 3′ splice sites produced wild-type levels of unspliced late RNA. In order to extend this work we have constructed additional types of mutants. Point mutations in the 5′ splice site confirmed its importance for RNA stability. Other mutants Included constructs in which the spacing between the 5′ splice site and the late promoter was altered and 5′ splice site insertion mutants where a 58 bp fragment containing the 5′ splice site sequence was inserted separately at various restriction sites in the late region. Both types of mutants lacked all of the late 3′ splice sites and had only a single 5′ splice site. RNase protection analyses of late and early RNAs from these constructs revealed that moving the 5′ splice site away from the late promoter (or from its normal context) destabilized late RNAs >10-fold relative to the wild-type. We conclude that both 5′ splice site integrity and its proximity to the late promoter play important roles in the nuclear stability of polyoma virus late RNAs.

This content is only available as a PDF.

Author notes

• Prescnt address: Epidemiology Program, Department of Public Health. Hartford, CT 06106, USA

© 1995 Oxford University Press

I agree to the terms and conditions. You must accept the terms and conditions.

Submit a comment

Name

Affiliations

Comment title

Comment

You have entered an invalid code

Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.

Citations

Views

Altmetric

Metrics

Total Views 39

12 Pageviews

27 PDF Downloads

Since 8/1/2017

Citations

8 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic