Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells (original) (raw)
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Department of Biochemistry and Molecular Biology, The University of Florida
Gainesville, FL 32610, USA
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Department of Immunology and Medical Microbiology, The University of Florida
Gainesville, FL 32610, USA
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Department of Biochemistry and Molecular Biology, The University of Florida
Gainesville, FL 32610, USA
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Received:
13 January 1978
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S. Detke, J.L. Stein, G.S. Stein, Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells, Nucleic Acids Research, Volume 5, Issue 5, 1 May 1978, Pages 1515–1528, https://doi.org/10.1093/nar/5.5.1515
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Abstract
Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of α-amanitin (1.0 μg/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
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