DNA sequence of the araC regulatory gene from Escherichia coli B/r (original) (raw)
Journal Article
,
Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles
Los Angeles, CA 90024, USA
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Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles
Los Angeles, CA 90024, USA
Search for other works by this author on:
,
Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles
Los Angeles, CA 90024, USA
Search for other works by this author on:
,
Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles
Los Angeles, CA 90024, USA
Search for other works by this author on:
Department of Microbiology and Molecular Biology Institute, University of California at Los Angeles
Los Angeles, CA 90024, USA
Search for other works by this author on:
Received:
08 September 1980
Published:
25 November 1980
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C.Garrett Miyada, Arnold H. Horwitz, Laura G. Cass, Josef Timko, Gary Wilcox, DNA sequence of the araC regulatory gene from Escherichia coli B/r, Nucleic Acids Research, Volume 8, Issue 22, 25 November 1980, Pages 5267–5274, https://doi.org/10.1093/nar/8.22.5267
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Abstract
The DNA sequence of the araC regulatory gene from Escherichiacoli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation In araC, Δ(araC0)719, removes all but the last 22 codons of the gene.
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