Promotion of specific in vitro transcription by excised “TATA” box sequences inserted in a foreign nucleotide environment (original) (raw)
Journal Article
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Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Institut de Chimie Biologique
11 Rue Humann, 67085 Strasbourg, France
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,
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Institut de Chimie Biologique
11 Rue Humann, 67085 Strasbourg, France
Search for other works by this author on:
,
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Institut de Chimie Biologique
11 Rue Humann, 67085 Strasbourg, France
Search for other works by this author on:
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Institut de Chimie Biologique
11 Rue Humann, 67085 Strasbourg, France
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Published:
25 August 1981
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P. Sassone-Corsi, J. Corden, C. Kédinger, P. Chambon, Promotion of specific in vitro transcription by excised “TATA” box sequences inserted in a foreign nucleotide environment, Nucleic Acids Research, Volume 9, Issue 16, 25 August 1981, Pages 3941–3958, https://doi.org/10.1093/nar/9.16.3941
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Abstract
We have cloned into plasmid pBR322 a DNA fragment extending from position −32 to position −12 of the adenovirus type 2 major late promoter region (position +1 referring to the cap site). In vitro transcription experiments show that this 21 base pair sequence, which contains the Goldberg-Hogness or “TATA” box, is both necessary and sufficient for specific initiation of transcription by RNA polymerase B (or II). Furthermore, we show that similar sequences, randomly occuring in the bacterial plasmid pBR322, are also recognized by the RNA polymerase B transcription machinery and able to promote specific in vitro transcription. Finally, we discuss the possible importance of the nucleotide sequence of the start region in the actual efficiency of initiation of in vitro transcription.
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© 1981 IRL Press Limited
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