The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29 (original) (raw)
Journal Article
,
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann
Ihnestrasse 63-73, Berlin-Dahlem, GFR
Search for other works by this author on:
,
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann
Ihnestrasse 63-73, Berlin-Dahlem, GFR
Search for other works by this author on:
,
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann
Ihnestrasse 63-73, Berlin-Dahlem, GFR
Search for other works by this author on:
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann
Ihnestrasse 63-73, Berlin-Dahlem, GFR
Search for other works by this author on:
Published:
11 September 1981
Cite
Iwona Wower, Jacek Wower, Marlis Meinke, Richard Brimacombe, The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29, Nucleic Acids Research, Volume 9, Issue 17, 11 September 1981, Pages 4285–4302, https://doi.org/10.1093/nar/9.17.4285
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
When E. coli ribosomal subunits are reacted with 2-iminothiolane and then subjected to a mild ultraviolet irradiation, an RNA-protein cross-linking reaction occurs. About 5% of the total protein in each subunit becomes cross-linked to the RNA, and a specific sub-set of proteins is involved in the reaction. In the case of the 50S subunit, the sites of cross-linking to the 23S RNA have been determined for six of these proteins: protein L4 is cross-linked within an oligonucleotide comprising positions 613–617 in the 23S sequence, L6 within positions 2473–2481, L21 within positions 540–548, L23 within positions 137–141, L27 within positions 2332–2337 and L29 within positions 99–107.
This content is only available as a PDF.
© IRL Press Limited
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 120
30 Pageviews
90 PDF Downloads
Since 2/1/2017
Month: | Total Views: |
---|---|
February 2017 | 1 |
May 2017 | 2 |
June 2017 | 1 |
August 2017 | 7 |
September 2017 | 5 |
October 2017 | 1 |
December 2017 | 9 |
January 2018 | 3 |
February 2018 | 3 |
March 2018 | 16 |
April 2018 | 6 |
May 2018 | 1 |
June 2018 | 1 |
September 2018 | 1 |
October 2018 | 1 |
August 2019 | 2 |
September 2019 | 1 |
October 2019 | 1 |
November 2019 | 2 |
March 2020 | 1 |
June 2020 | 1 |
August 2020 | 1 |
November 2020 | 2 |
December 2020 | 3 |
March 2021 | 1 |
September 2021 | 2 |
October 2021 | 1 |
November 2021 | 7 |
January 2022 | 1 |
August 2022 | 2 |
September 2022 | 1 |
November 2022 | 2 |
January 2023 | 2 |
June 2023 | 1 |
August 2023 | 1 |
September 2023 | 2 |
December 2023 | 1 |
April 2024 | 5 |
May 2024 | 1 |
June 2024 | 3 |
July 2024 | 4 |
August 2024 | 3 |
September 2024 | 2 |
October 2024 | 6 |
Citations
75 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic