There are approximately 20 actin genes in the human genome (original) (raw)
Journal Article
,
1
Department of Biochemistry, St. Mary's Hospital Medical School, University of London
London W2 1PG, UK
Search for other works by this author on:
,
1
Department of Biochemistry, St. Mary's Hospital Medical School, University of London
London W2 1PG, UK
Search for other works by this author on:
,
2
Department of Molecular Biology, Institut Pasteur
25 rue du Docteur Roux, 75015 Paris, France
Search for other works by this author on:
,
2
Department of Molecular Biology, Institut Pasteur
25 rue du Docteur Roux, 75015 Paris, France
Search for other works by this author on:
1
Department of Biochemistry, St. Mary's Hospital Medical School, University of London
London W2 1PG, UK
Search for other works by this author on:
Published:
10 October 1981
PDF Cite
S. E. Humphries, R. Whittall, A. Minty, M. Buckingham, R. Williamson, There are approximately 20 actin genes in the human genome, Nucleic Acids Research, Volume 9, Issue 19, 10 October 1981, Pages 4895–4908, https://doi.org/10.1093/nar/9.19.4895
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
By three different lines of evidence there are approximately 20 copies of actin genes in the human genome. Firstly, the rate of hybridisation of a mouse actin probe to human DNA indicates that there are a minimum of 20 complementary copies of the actin sequence per genome. Secondly, this probe hybridises to 17–20 bands in Southern blots of restriction enzyme digests of total human DNA. Most of these bands hybridise with both 3′ and 5′ fragments of the cDNA and are therefore likely to contain the entire gene sequence. Thirdly, we have picked 12 actin recombinants from a genomic library, and at the level of restriction enzyme mapping these represent nine different genes. Probability calculations indicate that these recombinants were picked from a pool of at least 20 different genes.
This content is only available as a PDF.
© 1981 IRL Press Limited
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 65
16 Pageviews
49 PDF Downloads
Since 2/1/2017
| Month: | Total Views: |
|---|---|
| February 2017 | 5 |
| May 2017 | 2 |
| August 2017 | 2 |
| October 2017 | 1 |
| December 2017 | 7 |
| January 2018 | 2 |
| February 2018 | 2 |
| March 2018 | 6 |
| April 2018 | 4 |
| August 2018 | 1 |
| July 2019 | 1 |
| November 2019 | 1 |
| September 2022 | 3 |
| October 2022 | 2 |
| November 2022 | 3 |
| December 2022 | 1 |
| January 2023 | 3 |
| March 2023 | 1 |
| May 2023 | 1 |
| September 2023 | 1 |
| February 2024 | 1 |
| April 2024 | 3 |
| May 2024 | 4 |
| June 2024 | 1 |
| July 2024 | 5 |
| August 2024 | 1 |
| September 2024 | 1 |
Citations
83 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic