Developmental Changes in Polyamine Levels and Synthesis in the Ovine Conceptus1 (original) (raw)

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3Department of Animal Science and Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843-2471

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3Department of Animal Science and Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843-2471

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3Department of Animal Science and Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843-2471

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3Department of Animal Science and Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, Texas 77843-2471

2Correspondence: Dr. Thomas E. Spencer, Center for Animal Biotechnology and Genomics, 442 Kleberg Center, 2471 TAMU, Texas A&M University, College Station, TX 77843-2471. FAX: 979 862 2662;

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Revision received:

29 May 2003

Published:

01 November 2003

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Hyukjung Kwon, Guoyao Wu, Fuller W. Bazer, Thomas E. Spencer, Developmental Changes in Polyamine Levels and Synthesis in the Ovine Conceptus, Biology of Reproduction, Volume 69, Issue 5, 1 November 2003, Pages 1626–1634, https://doi.org/10.1095/biolreprod.103.019067
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Abstract

Polyamines (putrescine, spermidine, and spermine) are essential for placental growth and angiogenesis. However, little is known about changes in polyamine synthesis associated with development of the ovine conceptus (embryo/fetus and associated placental membranes). We hypothesized that rates of placental polyamine synthesis were maximal during the rapid placental growth that occurs in the first half of pregnancy. This hypothesis was tested using ewes between Days 30 and 140 of gestation. Columbia cross-bred ewes were hysterectomized on Days 30, 40, 60, 80, 100, 120, or 140 of gestation (Day 0 = mating; n = 4 ewes/day) to obtain placentomes, intercotyledonary placenta, intercaruncular endometrium, and allantoic as well as amniotic fluids. The tissues were analyzed for ornithine decarboxylase (ODC) and arginase activities; arginine, ornithine, and polyamine concentrations; and polyamine synthesis using radiochemical and chromatographic methods. Maximal ODC and arginase activities and the highest rates of polyamine synthesis were observed in all tissues on Day 40 of gestation. Concentrations of ornithine and polyamines in placentomes and intercaruncular endometrium also peaked on Day 40 of gestation. In ovine allantoic and amniotic fluids, polyamines were most abundant during early (Days 40–60) and late (Days 100–140) gestation, respectively. Amniotic fluid spermine increased progressively with advancing gestation. Results of the present study indicate metabolic coordination among the several integrated pathways that support high rates of polyamine synthesis in the placenta and endometrium during early pregnancy. Our findings may have important implications for both intrauterine growth retardation and fetal origins of diseases in adults.

© 2003 by the Society for the Study of Reproduction, Inc.

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