Phenotypic Analysis of Four Human Medulloblastoma Cell Lines and Transplantable Xenografts (original) (raw)

Journal Article

,

Department of Pathology (Neuropathology),

Duke University

Medical Center, Durham, North Carolina

Search for other works by this author on:

,

Department of Pediatrics,

Duke University

Medical Center, Durham, North Carolina

Search for other works by this author on:

,

Carol J. Wikstrand, Ph.D.

Department of Pathology (Neuropathology),

Duke University

Medical Center, Durham, North Carolina

Search for other works by this author on:

,

Department of Pathology (Neuropathology),

Duke University

Medical Center, Durham, North Carolina

Department of Pediatrics,

Duke University

Medical Center, Durham, North Carolina

Search for other works by this author on:

,

John Q. Trojanowski, M.D., Ph.D

Department of Pathology, Division of Neuropathology,

University of Pennsylvania

, Philadelphia, Pennsylvania

Search for other works by this author on:

,

Oncology Laboratories,

Institute of Child Health

, London, United Kingdom

Search for other works by this author on:

,

Department of Neurosurgery,

Frenchay Hospital

, Frenchay, Bristol, United Kingdom

Search for other works by this author on:

,

Department of Pathology (Neuropathology),

Duke University

Medical Center, Durham, North Carolina

Search for other works by this author on:

Darell D. Bigner, M.D., Ph.D

Department of Pathology (Neuropathology),

Duke University

Medical Center, Durham, North Carolina

Department of the Preuss Laboratory for Brain Tumor Research,

Duke University

Medical Center, Durham, North Carolina

Correspondence to: Darell D. Bigner, M.D., Ph.D., P.O. Box 3156, Department of Pathology, Duke University Medical Center, Durham, NC 27710.

Search for other works by this author on:

Published:

01 January 1989

Cite

Xuanmin He, Stephen X. Skapek, Carol J. Wikstrand, Henry S. Friedman, John Q. Trojanowski, John T. Kemshead, Hugh B. Coakham, Sandra H. Bigner, Darell D. Bigner, Phenotypic Analysis of Four Human Medulloblastoma Cell Lines and Transplantable Xenografts, Journal of Neuropathology & Experimental Neurology, Volume 48, Issue 1, January 1989, Pages 48–68, https://doi.org/10.1097/00005072-198901000-00005
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

An extensive panel of monoclonal antibodies (MAb) and monospecific antisera reactive against neuroectodermal-, neuronal-, glial-, and lymphoid-associated antigens, extracellular matrix, HLA, and cell-surface receptors was used to characterize the phenotype of four continuous, karyotypically distinct medulloblastoma cell lines and transplantable xenografts. All four cell lines demonstrated significant reactivity with anti-neuroectodermal-associated MAb. No apparent pattern of reactivity with anti-lymphoid MAb was seen; notably, there was a uniform absence of detectable Thy-1. Review of the complete antibody reactivity profile revealed a dichotomy between lines TE-671 and Daoy and lines D283 Med and D341 Med, which have been previously shown to express neurofilament protein in culture and xenografts, and to exhibit neuroblastic morphological features in biopsy and xenograft tissue sections. TE-671 and Daoy reacted with the MAb directed against tenascin, epidermal growth factor (EGF) receptor, HLA-A,B epitopes, β2-microglobulin and 5/8 of the glioma-associated antigens, but did not react with the anti-neurofilament protein (NFP) MAb. D283 Med and D341 Med expressed NFP but did not react with MAb against tenascin, EGF receptor, HLA-A.B epitopes, β2-microglobulin or 6/8 and 7/8 (respectively) of the glioma-associated antigens. The observed phenotypic differences provide a conceptual framework for investigating basic differences in the biological behavior of medulloblastoma. Moreover, the subdivisions can be evaluated for prospective value in tissue diagnosis, cerebrospinal fluid cytology and antibody-mediated imaging and therapy.

This content is only available as a PDF.

© 1989, by the American Association of Neuropathologists

Topic:

You do not currently have access to this article.

Personal account

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

  1. Click Sign in through your institution.
  2. Select your institution from the list provided, which will take you to your institution's website to sign in.
  3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
  4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

  1. Click Sign in through society site.
  2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
  3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Phenotypic Analysis of Four Human Medulloblastoma Cell Lines and Transplantable Xenografts - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

Read this now at DeepDyve

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 22

2 Pageviews

20 PDF Downloads

Since 1/1/2017

Month: Total Views:
January 2017 3
February 2017 2
July 2018 1
June 2019 1
July 2019 1
December 2019 3
January 2020 1
April 2020 1
September 2020 1
November 2020 1
September 2021 1
April 2023 3
August 2023 1
October 2023 1
June 2024 1

Citations

85 Web of Science

×

Email alerts

Citing articles via

More from Oxford Academic