Phenotypic Analysis of Four Human Medulloblastoma Cell Lines and Transplantable Xenografts (original) (raw)
Journal Article
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Department of Pathology (Neuropathology),
Duke University
Medical Center, Durham, North Carolina
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Department of Pediatrics,
Duke University
Medical Center, Durham, North Carolina
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Carol J. Wikstrand, Ph.D.
Department of Pathology (Neuropathology),
Duke University
Medical Center, Durham, North Carolina
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Department of Pathology (Neuropathology),
Duke University
Medical Center, Durham, North Carolina
Department of Pediatrics,
Duke University
Medical Center, Durham, North Carolina
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John Q. Trojanowski, M.D., Ph.D
Department of Pathology, Division of Neuropathology,
University of Pennsylvania
, Philadelphia, Pennsylvania
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Oncology Laboratories,
Institute of Child Health
, London, United Kingdom
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Department of Neurosurgery,
Frenchay Hospital
, Frenchay, Bristol, United Kingdom
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Department of Pathology (Neuropathology),
Duke University
Medical Center, Durham, North Carolina
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Department of Pathology (Neuropathology),
Duke University
Medical Center, Durham, North Carolina
Department of the Preuss Laboratory for Brain Tumor Research,
Duke University
Medical Center, Durham, North Carolina
Correspondence to: Darell D. Bigner, M.D., Ph.D., P.O. Box 3156, Department of Pathology, Duke University Medical Center, Durham, NC 27710.
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Published:
01 January 1989
Cite
Xuanmin He, Stephen X. Skapek, Carol J. Wikstrand, Henry S. Friedman, John Q. Trojanowski, John T. Kemshead, Hugh B. Coakham, Sandra H. Bigner, Darell D. Bigner, Phenotypic Analysis of Four Human Medulloblastoma Cell Lines and Transplantable Xenografts, Journal of Neuropathology & Experimental Neurology, Volume 48, Issue 1, January 1989, Pages 48–68, https://doi.org/10.1097/00005072-198901000-00005
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Abstract
An extensive panel of monoclonal antibodies (MAb) and monospecific antisera reactive against neuroectodermal-, neuronal-, glial-, and lymphoid-associated antigens, extracellular matrix, HLA, and cell-surface receptors was used to characterize the phenotype of four continuous, karyotypically distinct medulloblastoma cell lines and transplantable xenografts. All four cell lines demonstrated significant reactivity with anti-neuroectodermal-associated MAb. No apparent pattern of reactivity with anti-lymphoid MAb was seen; notably, there was a uniform absence of detectable Thy-1. Review of the complete antibody reactivity profile revealed a dichotomy between lines TE-671 and Daoy and lines D283 Med and D341 Med, which have been previously shown to express neurofilament protein in culture and xenografts, and to exhibit neuroblastic morphological features in biopsy and xenograft tissue sections. TE-671 and Daoy reacted with the MAb directed against tenascin, epidermal growth factor (EGF) receptor, HLA-A,B epitopes, β2-microglobulin and 5/8 of the glioma-associated antigens, but did not react with the anti-neurofilament protein (NFP) MAb. D283 Med and D341 Med expressed NFP but did not react with MAb against tenascin, EGF receptor, HLA-A.B epitopes, β2-microglobulin or 6/8 and 7/8 (respectively) of the glioma-associated antigens. The observed phenotypic differences provide a conceptual framework for investigating basic differences in the biological behavior of medulloblastoma. Moreover, the subdivisions can be evaluated for prospective value in tissue diagnosis, cerebrospinal fluid cytology and antibody-mediated imaging and therapy.
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© 1989, by the American Association of Neuropathologists
Topic:
- phenotype
- extracellular matrix
- tenascin
- biopsy
- cytology
- human leukocyte antigens
- monoclonal antibodies
- antigens
- cell lines
- epidermal growth factor
- epitopes
- glioma
- hla-a antigens
- hla-a2 antigen
- immune sera
- medulloblastoma
- neurofilament proteins
- epidermal growth factor receptors
- receptors, cell surface
- transplantation, heterologous
- antibodies
- cerebrospinal fluid
- diagnosis
- diagnostic imaging
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