Proteomic Approach to Identify Novel Mitochondrial Proteins in Arabidopsis (original) (raw)

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2Applied Biosystems, D-63225 Langen, Germany (V.K.); and

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1Institut für Angewandte Genetik, Universität Hannover, D-30419 Hannover, Germany (H.E., W.W., H.-P.B.);

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3Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany (L.J.)

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1Institut für Angewandte Genetik, Universität Hannover, D-30419 Hannover, Germany (H.E., W.W., H.-P.B.);

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1Institut für Angewandte Genetik, Universität Hannover, D-30419 Hannover, Germany (H.E., W.W., H.-P.B.);

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Revision received:

20 August 2001

Accepted:

11 September 2001

Published:

01 December 2001

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Volker Kruft, Holger Eubel, Lothar Jänsch, Wolf Werhahn, Hans-Peter Braun, Proteomic Approach to Identify Novel Mitochondrial Proteins in Arabidopsis, Plant Physiology, Volume 127, Issue 4, December 2001, Pages 1694–1710, https://doi.org/10.1104/pp.010474
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Abstract

An Arabidopsis mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. Mitochondria were prepared from Arabidopsis stems and leaves or from Arabidopsis suspension cell cultures, and the purity of the generated fractions was tested by the resolution of organellar protein complexes applying two-dimensional blue-native/_N_-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine (Tricine) sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The Arabidopsis mitochondrial proteome was analyzed by two-dimensional isoelectric focusing/ Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 650 different proteins in a pI range of pH 3 to 10 were separated on single gels. Solubilization conditions, pH gradients for isoelectric focusing, and gel staining procedures were varied, and the number of separable proteins increased to about 800. Fifty-two protein spots were identified by immunoblotting, direct protein sequencing, and mass spectrometry. The characterized proteins cooperate in various processes, such as respiration, citric acid cycle, amino acid and nucleotide metabolism, protection against O2, mitochondrial assembly, molecular transport, and protein biosynthesis. More than 20% of the identified proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. The map of the Arabidopsis mitochondrial proteome should be useful for the analysis of knockout mutants concerning nuclear-encoded mitochondrial genes. Considerations of the total complexity of the Arabidopsis mitochondrial proteome are discussed. The data from this investigation will be made available athttp://www.gartenbau.uni-hannover.de/genetik/AMPP.

Copyright © 2001 American Society of Plant Physiologists

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