Characterization of Glucose-6-Phosphate Incorporation into Starch by Isolated Intact Cauliflower-Bud Plastids (original) (raw)

Journal Article

,

Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universitat Osnabruck, Barbarastrasse 11, D-4500 Osnabruck, Federal Republic of Germany

Search for other works by this author on:

,

Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universitat Osnabruck, Barbarastrasse 11, D-4500 Osnabruck, Federal Republic of Germany

Search for other works by this author on:

Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universitat Osnabruck, Barbarastrasse 11, D-4500 Osnabruck, Federal Republic of Germany

Search for other works by this author on:

Published:

01 February 1993

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

H. E. Neuhaus, G. Henrichs, R. Scheibe, Characterization of Glucose-6-Phosphate Incorporation into Starch by Isolated Intact Cauliflower-Bud Plastids, Plant Physiology, Volume 101, Issue 2, February 1993, Pages 573–578, https://doi.org/10.1104/pp.101.2.573
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Intact plastids from cauliflower (Brassica oleracea var Prince de Bretagne) buds were isolated according to the method described by Journet and Douce (E.P. Journet and R. Douce [1985] Plant Physiol 79: 458–)467). Incubation of these plastids with various 14C-labeled compounds revealed that glucose-6-phosphate can act as a precursor for starch synthesis. However, significant rates (incorporation of 120 nmol glucose mg-1 protein h-1) could only be observed when both 3-phosphoglyceric acid and ATP were present as well. Starch synthesis in isolated plastids was strongly dependent upon the intactness of the organelle. The presence of a high-affinity ATP/ADP translocator with a Km for ATP of 12 [mu]M was demonstrated by uptake experiments with [14C]ATP. ADP inhibited both ATP uptake and effector-stimulated starch synthesis. Effector-stimulated glucose-6-phosphate-dependent starch synthesis was not significantly influenced by fructose-6-phosphate or 2-deoxyglucose-6-phosphate but was strongly inhibited by triose phosphate and inorganic phosphate. Starch synthesis was also inhibited by 4,4[prime]-diisothio-cyanostilbene-2,2[prime]-disulfonate, which is known to be a potent inhibitor of the chloroplast phosphate translocator. The data presented here support the view that starch biosynthesis in heterotrophic tissues is powered by increasing levels of cytosolic 3-phosphoglyceric acid and ATP when glucose-6-phosphate is available.

This content is only available as a PDF.

Copyright © 1993 by American Society of Plant Biologists

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

Citations

Views

Altmetric

Metrics

Total Views 67

0 Pageviews

67 PDF Downloads

Since 1/1/2021

Citations

59 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic