Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans 1 (original) (raw)
Journal Article
,
Karl-Heinrich Fritzemeier
Search for other works by this author on:
,
Search for other works by this author on:
,
Search for other works by this author on:
,
Search for other works by this author on:
,
Search for other works by this author on:
,
Search for other works by this author on:
Search for other works by this author on:
Published:
01 September 1987
PDF Cite
Karl-Heinrich Fritzemeier, Claude Cretin, Erich Kombrink, Frauke Rohwer, Janet Taylor, Dierk Scheel, Klaus Hahlbrock, Transient Induction of Phenylalanine Ammonia-Lyase and 4-Coumarate: CoA Ligase mRNAs in Potato Leaves Infected with Virulent or Avirulent Races of Phytophthora infestans , Plant Physiology, Volume 85, Issue 1, September 1987, Pages 34–41, https://doi.org/10.1104/pp.85.1.34
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Infection of potato leaves with the fungal pathogen Phytophthora infestans (Pi) resulted in the rapid stimulation of phenylpropanoid metabolism. Increases in the activities of several mRNAs, including those encoding phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL), were detectable within a few hours postinoculation, as demonstrated by two-dimensional gel electrophoresis of proteins synthesized in vitro. This effect was closely mimicked by application of Pi culture filtrate through cut leaf stems. PAL and 4CL mRNA activities were also rapidly and transiently induced in potato cell suspension cultures by treatments with Pi culture filtrate or arachidonic acid. This induction was exploited to generate cDNA probes complementary to PAL and 4CL mRNAs. Blot hybridizations using these probes revealed almost immediate, transient and coordinate increases in the transcription rates and subsequent changes in the amounts of PAL and 4CL mRNAs in leaves treated with Pi culture filtrate. Similar changes in the mRNA amounts were found in infected leaves of potato cultivars carrying resistance genes R1 (cv Datura) or R4 (cv Isola), independent of whether a virulent or an avirulent Pi pathotype was used for inoculation. These results are discussed in relation to recent cytological observations with the same potato cultivars and Pi pathotypes.
1
Supported by the Bundesministerium für Forschung und Technologie, FRG.
This content is only available as a PDF.
© 1987 American Society of Plant Biologists
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)
Citations
Views
Altmetric
Metrics
Total Views 66
0 Pageviews
66 PDF Downloads
Since 4/1/2021
| Month: | Total Views: |
|---|---|
| April 2021 | 1 |
| August 2021 | 3 |
| September 2021 | 2 |
| October 2021 | 8 |
| November 2021 | 1 |
| January 2022 | 2 |
| February 2022 | 2 |
| March 2022 | 1 |
| April 2022 | 1 |
| May 2022 | 4 |
| June 2022 | 1 |
| July 2022 | 1 |
| August 2022 | 1 |
| September 2022 | 2 |
| October 2022 | 2 |
| November 2022 | 1 |
| January 2023 | 2 |
| February 2023 | 5 |
| May 2023 | 4 |
| June 2023 | 2 |
| August 2023 | 1 |
| September 2023 | 3 |
| October 2023 | 1 |
| November 2023 | 1 |
| December 2023 | 1 |
| January 2024 | 1 |
| February 2024 | 3 |
| March 2024 | 1 |
| April 2024 | 1 |
| May 2024 | 1 |
| July 2024 | 3 |
| August 2024 | 1 |
| September 2024 | 2 |
Citations
122 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic