A Novel cis-Acting Element in Promoters of Plant B-Type Cyclin Genes Activates M Phase–Specific Transcription (original) (raw)
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a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan
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a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan
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b Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-81, Japan
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a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan
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c Department of Chemical and Biological Sciences, Japan Women’s University, Mejirodai, Tokyo 112, Japan
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d Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-01, Japan
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d Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-01, Japan
e Department of Plant Science, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-01, Japan
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a Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113, Japan
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Received:
17 October 1997
Accepted:
19 January 1998
Cite
Masaki Ito, Masayuki Iwase, Hiroaki Kodama, Philippe Lavisse, Atsushi Komamine, Ryuichi Nishihama, Yasunori Machida, Akira Watanabe, A Novel _cis_-Acting Element in Promoters of Plant B-Type Cyclin Genes Activates M Phase–Specific Transcription, The Plant Cell, Volume 10, Issue 3, March 1998, Pages 331–341, https://doi.org/10.1105/tpc.10.3.331
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Abstract
Plant B-type cyclin genes are expressed late in the G2 and M phases of the cell cycle. Previously, we showed that the promoter of a Catharanthus roseus B-type cyclin, CYM, could direct M phase–specific transcription of a β-glucuronidase reporter gene in synchronously dividing BY2 tobacco cells. In this study, we determined the regulatory elements contained within the CYM promoter by using a luciferase reporter gene. Mutational analysis showed that a 9-bp element is essential for M phase–specific promoter activity in synchronized BY2 cells. The CYM promoter contains three other sequences similar to this element. A gain-of-function assay demonstrated that when fused to a heterologous promoter, these elements are sufficient for M phase–specific expression; therefore, we named these elements M-specific activators (MSAs). We found MSA-like sequences in B-type cyclin promoters from tobacco, soybean, and Arabidopsis as well as in the promoters of two M phase–specific genes, NACK1 and NACK2, which encode tobacco kinesin-like proteins. Thus, MSA may be a common _cis_-acting promoter element that controls M phase–specific expression of cell cycle–related genes in plants.
© 1998 American Society of Plant Physiologists
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