Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings. (original) (raw)

Journal Article

,

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

,

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

,

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

,

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

,

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.

Search for other works by this author on:

Published:

01 October 1992

• PDF
• Split View
• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

W. L. Kubasek, B. W. Shirley, A. McKillop, H. M. Goodman, W. Briggs, F. M. Ausubel, Regulation of Flavonoid Biosynthetic Genes in Germinating Arabidopsis Seedlings., The Plant Cell, Volume 4, Issue 10, October 1992, Pages 1229–1236, https://doi.org/10.1105/tpc.4.10.1229
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Many higher plants, including Arabidopsis, transiently display purple anthocyanin pigments just after seed germination. We observed that steady state levels of mRNAs encoded by four flavonoid biosynthetic genes, PAL1 (encoding phenylalanine ammonia-lyase 1), CHS (encoding chalcone synthase), CHI (encoding chalcone isomerase), and DFR (encoding dihydroflavonol reductase), were temporally regulated, peaking in 3-day-old seedlings grown in continuous white light. Except for the case of PAL1 mRNA, mRNA levels for these flavonoid genes were very low in seedlings grown in darkness. Light induction studies using seedlings grown in darkness showed that PAL1 mRNA began to accumulate before CHS and CHI mRNAs, which, in turn, began to accumulate before DFR mRNA. This order of induction is the same as the order of the biosynthetic steps in flavonoid biosynthesis. Our results suggest that the flavonoid biosynthetic pathway is coordinately regulated by a developmental timing mechanism during germination. Blue light and UVB light induction experiments using red light- and dark-grown seedlings showed that the flavonoid biosynthetic genes are induced most effectively by UVB light and that blue light induction is mediated by a specific blue light receptor.

This content is only available as a PDF.

© 1992 by American Society of Plant Biologists

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

Citations

Views

Altmetric

Metrics

Total Views 319

0 Pageviews

319 PDF Downloads

Since 3/1/2021

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic