Identification and characterization of specific sequences encoding pathogenicity associated proteins in the genome of commensal Neisseria species (original) (raw)

Journal Article

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Department of Biotechnology, TB-Z, Boehringer Mannheim GmbH, Nonnenwald 2, 82377 Penzberg, Germany

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Department of Biotechnology, TB-Z, Boehringer Mannheim GmbH, Nonnenwald 2, 82377 Penzberg, Germany

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Received:

29 September 1994

Revision received:

12 November 1994

Accepted:

18 November 1994

Published:

01 January 1995

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Karin Wolff, Anne Stern, Identification and characterization of specific sequences encoding pathogenicity associated proteins in the genome of commensal Neisseria species, FEMS Microbiology Letters, Volume 125, Issue 2-3, January 1995, Pages 255–263, https://doi.org/10.1111/j.1574-6968.1995.tb07366.x
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Abstract

The distribution of distinct sequences in pathogenic and commensal Neisseria species was investigated systematically by dot blot analysis. Probes representing the genes of Rmp, pilin and IgA1 protease were found to hybridize exclusively to the chromosomal DNA of the pathogenic species, Neisseria gonorrhoeae and/or Neisseria meningitidis. In contrast, specific sequences for the genes of the porin protein Por and the opacity protein (Opa) were also detected in a panel of commensal Neisseria species such as N. lactamica, N. subflava, N, flava, N. mucosa and N. sicca. Using _opa_-specific oligonucleotides as probes in chromosomal blots, the genomes of the commensal Neisseria species show a totally reduced repertoire of cross-hybridizing loci compared to the complex opa gene family of N. gonorrhoeae. DNA sequence analysis of one _opa_-related gene derived from N. flava and N. sicca, respectively, revealed a large degree of homology with previously described gonococcal and meningococcal genes e.g., a typical repetitive sequence in the leader peptide and the distribution of the hypervariable and conserved regions. This observation, together with the finding, that the gene is constitutively transcribed, leads to the assumption that some of the commensal Neisseria species may have the potential for the expression of a protein harboring similar functions as the Opa proteins in pathogenic Neisseriae.

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