The Dynamic Response of Vascular Endothelial Cells to Fluid Shear Stress (original) (raw)
Research Papers
Fluid Mechanics Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Mass. 02139
Search for other works by this author on:
Fluid Mechanics Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Mass. 02139
Search for other works by this author on:
Vascular Pathophysiology Laboratory, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Mass. 02115
Search for other works by this author on:
Vascular Pathophysiology Laboratory, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Mass. 02115
Search for other works by this author on:
C. F. Dewey, Jr.
Fluid Mechanics Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Mass. 02139
S. R. Bussolari
Fluid Mechanics Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Mass. 02139
M. A. Gimbrone, Jr.
Vascular Pathophysiology Laboratory, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Mass. 02115
P. F. Davies
Vascular Pathophysiology Laboratory, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, Mass. 02115
J Biomech Eng. Aug 1981, 103(3): 177-185 (9 pages)
Published Online: August 1, 1981
Citation
Dewey, C. F., Jr., Bussolari, S. R., Gimbrone, M. A., Jr., and Davies, P. F. (August 1, 1981). "The Dynamic Response of Vascular Endothelial Cells to Fluid Shear Stress." ASME. J Biomech Eng. August 1981; 103(3): 177–185. https://doi.org/10.1115/1.3138276
Download citation file:
- Ris (Zotero)
- Reference Manager
- EasyBib
- Bookends
- Mendeley
- Papers
- EndNote
- RefWorks
- BibTex
- ProCite
- Medlars
We have developed an in-vitro system for studying the dynamic response of vascular endothelial cells to controlled levels of fluid shear stress. Cultured monolayers of bovine aortic endothelial cells are placed in a cone-plate apparatus that produces a uniform fluid shear stress on replicate samples. Subconfluent endothelial cultures continuously exposed to 1–5 dynes/cm2 shear proliferate at a rate comparable to that of static cultures and reach the same saturation density (≃ 1.0–1.5 × 105 cells/cm2). When exposed to a laminar shear stress of 5–10 dynes/cm2, confluent monolayers undergo a time-dependent change in cell shape from polygonal to ellipsoidal and become uniformly oriented with flow. Regeneration of linear “wounds” in confluent monolayer appears to be influenced by the direction of the applied force. Preliminary studies indicate that certain endothelial cell functions, including fluid endocytosis, cytoskeletal assembly and nonthrombogenic surface properties, also are sensitive to shear stress. These observations suggest that fluid mechanical forces can directly influence endothelial cell structure and function. Modulation of endothelial behavior by fluid shear stresses may be relevant to normal vessel wall physiology, as well as the pathogenesis of vascular diseases, such as atherosclerosis.
Topics:
[Dynamic response](/biomechanical/search-results?f%5FSemanticFilterTopics=Dynamic response), [Endothelial cells](/biomechanical/search-results?f%5FSemanticFilterTopics=Endothelial cells), Fluids, [Shear stress](/biomechanical/search-results?f%5FSemanticFilterTopics=Shear stress), Atherosclerosis, Density, Diseases, [Flow (Dynamics)](/biomechanical/search-results?f%5FSemanticFilterTopics=Flow %28Dynamics%29), [Fluid mechanics](/biomechanical/search-results?f%5FSemanticFilterTopics=Fluid mechanics), Manufacturing, Physiology, Shapes, [Shear (Mechanics)](/biomechanical/search-results?f%5FSemanticFilterTopics=Shear %28Mechanics%29), [Surface properties](/biomechanical/search-results?f%5FSemanticFilterTopics=Surface properties), Vessels, Wounds
This content is only available via PDF.
You do not currently have access to this content.
Sign In
Purchase this Content
898 Views
940 Web of Science
Get Email Alerts
Cited By
Related Articles
Related Proceedings Papers
Related Chapters
Mechanical Blood Trauma in Circulatory-Assist Devices