Expression of the Growth Hormone/Insulin-Like Growth Factor Axis during Balb/c Thymus Ontogeny and Effects of Growth Hormone upon ex vivo T Cell Differentiation (original) (raw)
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Research Articles| January 18 2012
aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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cLaboratory of Experimental Medicine and Endocrinology, Catholic University of Leuven, Leuven, Belgium
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bDepartment of Hematology, University of Liège, CHU-B23, Liège-Sart Tilman, and
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bDepartment of Hematology, University of Liège, CHU-B23, Liège-Sart Tilman, and
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aCenter of Immunology, Institute of Pathology, and
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aCenter of Immunology, Institute of Pathology, and
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Neuroimmunomodulation (2012) 19 (3): 137–147.
Received:
January 19 2011
Published Online:
January 18 2012
Citation
Hamid Kermani, Lindsay Goffinet, Marie Mottet, Gwenaelle Bodart, Gabriel Morrhaye, Olivier Dardenne, Chantal Renard, Lut Overbergh, Frédéric Baron, Yves Beguin, Vincent Geenen, Henri J. Martens; Expression of the Growth Hormone/Insulin-Like Growth Factor Axis during Balb/c Thymus Ontogeny and Effects of Growth Hormone upon ex vivo T Cell Differentiation. _Neuroimmunomodulation 1 March 2012; 19 (3): 137–147. https://doi.org/10.1159/000328844
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Abstract
Aims: We address the question of the expression and the role of the growth hormone/insulin-like growth factor (GH/IGF) axis in the thymus. Methods: Using RT-qPCR, the expression profile of various components of the somatotrope GH/IGF axis was measured in different thymic cell types and during thymus embryogenesis in Balb/c mice. The effect of GH on T cell differentiation was explored via thymic organotypic culture. Results: Transcription of Gh, Igf1, Igf2 and their related receptors predominantly occurred in thymic epithelial cells (TEC), while a low level of Gh and Igf1r transcription was also evidenced in thymic T cells (thymocytes). Gh, Ghr, Ins2, Igf1, Igf2, and Igfr1 displayed distinct expression profiles depending on the developmental stage. The protein concentrations of IGF-1 and IGF-2 were in accordance with the profile of their gene expression. In fetal thymus organ cultures (FTOC) derived from Balb/c mice, treatment with exogenous GH resulted in a significant increase of double negative CD4–CD8– T cells and CD4+ T cells, together with a decrease in double positive CD4+CD8+ T cells. These changes were inhibited by concomitant treatment with GH and the GH receptor (GHR) antagonist pegvisomant. However, GH treatment also induced a significant decrease in FTOC Gh, Ghr and Igf1 expression. Conclusion: These data show that the thymotropic properties of the somatotrope GH/IGF-1 axis involve an interaction between exogenous GH and GHR expressed by TEC. Since thymic IGF-1 is not increased by GH treatment, the effects of GH upon T cell differentiation could implicate a different local growth factor or cytokine.
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© 2012 S. Karger AG, Basel
2012
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