Keratinocyte growth factor induces proliferation of hepatocytes and epithelial cells throughout the rat gastrointestinal tract. (original) (raw)
Research Article Free access | 10.1172/JCI117524
C F Morris, W Boyle, B Ring, R Biltz, J E Tarpley, S L Aukerman, P L Devine, R H Whitehead, and G F Pierce
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Housley, R. in:[JCI](/search/results?q=author.first%5Fname%3A%22R M%22+author.last%5Fname%3A%22Housley%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Morris, C. in:[JCI](/search/results?q=author.first%5Fname%3A%22C F%22+author.last%5Fname%3A%22Morris%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Boyle, W. in:JCI |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Ring, B. in:JCI |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Biltz, R. in:JCI |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Tarpley, J. in:[JCI](/search/results?q=author.first%5Fname%3A%22J E%22+author.last%5Fname%3A%22Tarpley%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Aukerman, S. in:[JCI](/search/results?q=author.first%5Fname%3A%22S L%22+author.last%5Fname%3A%22Aukerman%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Devine, P. in:[JCI](/search/results?q=author.first%5Fname%3A%22P L%22+author.last%5Fname%3A%22Devine%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Whitehead, R. in:[JCI](/search/results?q=author.first%5Fname%3A%22R H%22+author.last%5Fname%3A%22Whitehead%22&search%5Ftype=advanced) |PubMed |Google Scholar
Department of Laboratory Animal Resources, Amgen Inc., Thousand Oaks, California 91320.
Find articles by Pierce, G. in:[JCI](/search/results?q=author.first%5Fname%3A%22G F%22+author.last%5Fname%3A%22Pierce%22&search%5Ftype=advanced) |PubMed |Google Scholar
Published November 1, 1994 -More info
Published November 1, 1994 -Version history
Keratinocyte growth factor (KGF), a member of the fibroblast growth factor (FGF) family, was identified as a specific keratinocyte mitogen after isolation from a lung fibroblast line. Recently, recombinant (r)KGF was found to influence proliferation and differentiation patterns of multiple epithelial cell lineages within skin, lung, and the reproductive tract. In the present study, we designed experiments to identify additional target tissues, and focused on the rat gastrointestinal (GI) system, since a putative receptor, K-sam, was originally identified in a gastric carcinoma. Expression of KGF receptor and KGF mRNA was detected within the entire GI tract, suggesting the gut both synthesized and responded to KGF. Therefore, rKGF was administered to adult rats and was found to induce markedly increased proliferation of epithelial cells from the foregut to the colon, and of hepatocytes, one day after systemic treatment. Daily treatment resulted in the marked selective induction of mucin-producing cell lineages throughout the GI tract in a dose-dependent fashion. Other cell lineages were either unaffected (e.g., Paneth cells), or relatively decreased (e.g., parietal cells, enterocytes) in rKGF-treated rats. The direct effect of rKGF was confirmed by demonstrating markedly increased carcinoembryonic antigen production in a human colon carcinoma cell line, LIM1899. Serum levels of albumin were specifically and significantly elevated after daily treatment. These results demonstrate rKGF can induce epithelial cell activation throughout the GI tract and liver. Further, endogenous KGF may be a normal paracrine mediator of growth within the gut.
Images.
Click on an image below to see the page. View PDF of the complete article
- Version 1 (November 1, 1994): No description