Biosynthesis of silver nanoparticles using Salmonella typhirium (original) (raw)

  1. Journal of Nanostructure in Chemistry

  2. Vol. 3 No. 1 (December 2013)

  3. Original

  4. Department of Chemical engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IR

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Published in Issue 03-05-2013

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Abstract

Abstract The use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach. In the present investigation, we report the biosynthesis of silver nanoparticles employing the bacterium Salmonella typhirium . Silver nanoparticles were synthesized through the reduction of aqueous Ag+ ion using the growth culture supernatants in the bright conditions. The synthetic process was fast, and silver nanoparticles were formed within 4 min of silver ion coming in contact with the cell filtrate. An absorption peak at 427 nm in a Uv-vis spectrophotometer was detected indicating the presence of Ag nanoparticles. The morphology of nanoparticles was observed by transmission electron microscopy. The size of the nanoparticles was determined to be 87 ± 30 nm, applying dynamic light scattering. Reduction degree of Ag+ was measured as 75% by atomic absorption spectrophotometry. The silver nanoparticles have been prepared from silver sulfate during this investigation for the first time.

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References

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