Chondroitin sulfate disaccharide stimulates microglia to adopt a novel regulatory phenotype (original) (raw)

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Institute of Human Genetics, University of Regensburg

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Regensburg

,

Germany

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Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

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,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

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Center of Excellence for Fluorescent Bioanalytics

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Regensburg

,

Germany

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,

Center of Excellence for Fluorescent Bioanalytics

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Regensburg

,

Germany

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Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

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Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

Received:

26 February 2008

Revision received:

22 April 2008

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Stefanie Ebert, Tobias Schoeberl, Yana Walczak, Katharina Stoecker, Thomas Stempfl, Christoph Moehle, Bernhard H F Weber, Thomas Langmann, Chondroitin sulfate disaccharide stimulates microglia to adopt a novel regulatory phenotype, Journal of Leukocyte Biology, Volume 84, Issue 3, Sep 2008, Pages 736–740, https://doi.org/10.1189/jlb.0208138
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Abstract

A disaccharide degradation product of chondrotin sulfate proteoglycan-disaccharide (CSPG-DS) has been implicated previously in the inhibition of neurodegeneration by influencing microglia activation. In this study, genome-wide microarray analysis was used to identify specific gene expression profiles of CSPG-DS-stimulated BV-2 microglia-like cells. Gene products involved in phagocytosis, detoxification, migration, immune regulation, and antigen presentation were found to be altered significantly. These findings were replicated and compared with IFN-γ-stimulated primary microglia using real-time quantitative RT-PCR validation. Importantly, a unique transcriptional phenotype with anti-inflammatory and IFN-γ counter-regulatory properties partially related to alternatively activated macrophages was identified. Using functional cell assays, we found that CSPG-DS-stimulated microglia possess increased phagocytic capacity but lack direct cytotoxic effects such as secretion of NO. Furthermore, conditioned media from CSPG-DS-treated microglia did not diminish the viability or cause apoptosis of cultured photoreceptor cells and partially rescued these cells from IFN-γ-induced apoptosis. Taken together, our data provide a unique transcript dataset and important in vitro findings about the functional properties of CSPG-DS-activated microglia. These might be starting points to explore the in vivo role of CSPG-DS as a bioactive microglia regulator and its potential, therapeutic application in immune-related, neurodegenerative disorders.

© 2008 Society for Leukocyte Biology

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