Chondroitin sulfate disaccharide stimulates microglia to adopt a novel regulatory phenotype (original) (raw)

Journal Article

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

,

Center of Excellence for Fluorescent Bioanalytics

,

Regensburg

,

Germany

Search for other works by this author on:

,

Center of Excellence for Fluorescent Bioanalytics

,

Regensburg

,

Germany

Search for other works by this author on:

,

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

Institute of Human Genetics, University of Regensburg

,

Regensburg

,

Germany

Search for other works by this author on:

Received:

26 February 2008

Revision received:

22 April 2008

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Stefanie Ebert, Tobias Schoeberl, Yana Walczak, Katharina Stoecker, Thomas Stempfl, Christoph Moehle, Bernhard H F Weber, Thomas Langmann, Chondroitin sulfate disaccharide stimulates microglia to adopt a novel regulatory phenotype, Journal of Leukocyte Biology, Volume 84, Issue 3, Sep 2008, Pages 736–740, https://doi.org/10.1189/jlb.0208138
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

A disaccharide degradation product of chondrotin sulfate proteoglycan-disaccharide (CSPG-DS) has been implicated previously in the inhibition of neurodegeneration by influencing microglia activation. In this study, genome-wide microarray analysis was used to identify specific gene expression profiles of CSPG-DS-stimulated BV-2 microglia-like cells. Gene products involved in phagocytosis, detoxification, migration, immune regulation, and antigen presentation were found to be altered significantly. These findings were replicated and compared with IFN-γ-stimulated primary microglia using real-time quantitative RT-PCR validation. Importantly, a unique transcriptional phenotype with anti-inflammatory and IFN-γ counter-regulatory properties partially related to alternatively activated macrophages was identified. Using functional cell assays, we found that CSPG-DS-stimulated microglia possess increased phagocytic capacity but lack direct cytotoxic effects such as secretion of NO. Furthermore, conditioned media from CSPG-DS-treated microglia did not diminish the viability or cause apoptosis of cultured photoreceptor cells and partially rescued these cells from IFN-γ-induced apoptosis. Taken together, our data provide a unique transcript dataset and important in vitro findings about the functional properties of CSPG-DS-activated microglia. These might be starting points to explore the in vivo role of CSPG-DS as a bioactive microglia regulator and its potential, therapeutic application in immune-related, neurodegenerative disorders.

© 2008 Society for Leukocyte Biology

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Chondroitin sulfate disaccharide stimulates microglia to adopt a novel regulatory phenotype - 24 Hours access

EUR €48.00

GBP £41.00

USD $51.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 103

67 Pageviews

36 PDF Downloads

Since 2/1/2023

Citations

36 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic