Plasminogen activator inhibitor-2 (PAI-2) in eosinophilic leukocytes (original) (raw)

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Laboratorie of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

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Laboratorie of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

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Laboratorie of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

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Laboratorie of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

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Laboratorie of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

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Laboratorie of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

, Bethesda, Maryland

Correspondence: Building 10, Room 11N104, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892. E-mail: hrosenberg@niaid.nih.gov

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Revision received:

23 June 2004

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Jonathan M Swartz, Jonas Byström, Kimberly D Dyer, Takeaki Nitto, Thomas A Wynn, Helene F Rosenberg, Plasminogen activator inhibitor-2 (PAI-2) in eosinophilic leukocytes, Journal of Leukocyte Biology, Volume 76, Issue 4, Oct 2004, Pages 812–819, https://doi.org/10.1189/jlb.0304182
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Abstract

Plasminogen activator inhibitor-2 (PAI-2) as a potential eosinophil protein was inferred from our gene microarray study of mouse eosinophilopoiesis. Here, we detect 47 kDa intracellular and ∼60 kDa secretory forms of PAI-2 in purified human eosinophil extracts. PAI-2 is present at variable concentrations in eosinophil lysates, ranging from 30 to 444 ng/106 cells, with a mean of 182 ng/106 cells from 10 normal donors, which is the highest per-cell concentration among all leukocyte subtypes evaluated. Enzymatic assay confirmed that eosinophil-derived PAI-2 is biologically active and inhibits activation of its preferred substrate, urokinase. Immunohistochemical and immunogold staining demonstrated PAI-2 localization in eosinophil-specific granules. Immunoreactive PAI-2 was detected in extracellular deposits in and around the eosinophil-enriched granuloma tissue encapsulating the parasitic egg in livers of wild-type mice infected with the helminthic parasite Schistosoma mansoni. Among the possibilities, we consider a role for eosinophil-derived PAI-2 in inflammation and remodeling associated with parasitic infection as well as allergic airways disease, respiratory virus infection, and host responses to tumors and metastasis in vivo.

© 2004 Society for Leukocyte Biology

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