Irisin, a Novel Myokine, Regulates Glucose Uptake in Skeletal Muscle Cells via AMPK (original) (raw)
Journal Article
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
Nami Kim ,
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
1Department of Anatomy (H.J.L., J.O.L., N.K., J.K.K., H.I.K., Y.W.L., S.J.K., S.H.P., H.S.K.), Korea University College of Medicine, Seoul, Korea 136-705
Search for other works by this author on:
2Division of Cardiology (J.-I.C.), Department of Internal Medicine, Korea University Medical Center, Seoul, Korea
Search for other works by this author on:
3College of Nursing (Y.O.), Korea University, Seoul, Korea 136-705
Search for other works by this author on:
4College of Pre-Pharm·Med (J.H.K.), DukSung Women's University, Seoul, Korea 132-714
Search for other works by this author on:
Received:
05 November 2014
Cite
Hye Jeong Lee, Jung Ok Lee, Nami Kim, Joong Kwan Kim, Hyung Ip Kim, Yong Woo Lee, Su Jin Kim, Jong-Il Choi, Yoonji Oh, Jeong Hyun Kim, Suyeon- Hwang, Sun Hwa Park, Hyeon Soo Kim, Irisin, a Novel Myokine, Regulates Glucose Uptake in Skeletal Muscle Cells via AMPK, Molecular Endocrinology, Volume 29, Issue 6, 1 June 2015, Pages 873–881, https://doi.org/10.1210/me.2014-1353
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Irisin is a novel myokine produced by skeletal muscle. However, its metabolic role is poorly understood. In the present study, irisin induced glucose uptake in differentiated skeletal muscle cells. It increased AMP-activated protein kinase (AMPK) phosphorylation and the inhibition of AMPK blocked glucose uptake. It also increased reactive oxygen species (ROS) generation. N-acetyl cysteine, a ROS scavenger, blocked irisin-induced AMPK phosphorylation. Moreover, irisin activated p38 MAPK in an AMPK-dependent manner. The inhibition and knockdown of p38 MAPK blocked irisin-induced glucose uptake. A colorimetric absorbance assay showed that irisin stimulated the translocation of glucose transporter type 4 to the plasma membrane and that this effect was suppressed in cells pretreated with a p38 MAPK inhibitor or p38 MAPK small interfering RNA. In primary cultured myoblast cells, irisin increased the concentration of intracellular calcium. STO-609, a calcium/calmodulin-dependent protein kinase kinase inhibitor, blocked irisin-induced AMPK phosphorylation, implying that calcium is involved in irisin-mediated signaling. Our results suggest that irisin plays an important role in glucose metabolism via the ROS-mediated AMPK pathway in skeletal muscle cells.
Copyright © 2015 by the Endocrine Society
You do not currently have access to this article.
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution's website and Oxford Academic.
- Click Sign in through your institution.
- Select your institution from the list provided, which will take you to your institution's website to sign in.
- When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If your institution is not listed or you cannot sign in to your institution's website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see "Sign in through society site" in the sign in pane within a journal:
- Click Sign in through society site.
- When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
- View your signed in personal account and access account management features.
- View the institutional accounts that are providing access.
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.
Rental
This article is also available for rental through DeepDyve.
Citations
Views
Altmetric
Email alerts
Related articles in PubMed
Citing articles via
More from Oxford Academic