Isolation of TSH and LH/CG Receptor cDNAs from Human Thyroid: Regulation by Tissue Specific Splicing (original) (raw)
Journal Article
,
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
,
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
,
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
,
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
,
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
1Division of Molecular Medicine, New England Medical Center Hospital Boston, Massachusetts 02111; Department of Pathology, New England Medical Center Hospital Boston, Massachusetts 02111; Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University Portland, Oregon 97201; Division of Hematology/Oncology, The Children's Hospital Boston, Massachusetts; Zentrum for Molekulare Biologie Heidelberg, University of Heidelberg Heidelberg, FRG; The Population Council New York, New York 10021
Search for other works by this author on:
This work was supported by NIH Grants DK-41921-01 and the Medical Research Foundation (to R.D.C.) and by NIH Grant HD-22196(to D.L.S.).
A.L.F. was supported by a fellowship from the Association for Brain Tumor Research.
Present address: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland Oregon 97201; to whom requests for reprints should be addressed.
Revision received:
29 April 1990
Published:
01 August 1990
PDF Cite
A. Lindsay Frazier, Linda S. Robbins, Philip J. Stork, Rolf Sprengel, Deborah L. Segaloff, Roger D. Cone, Isolation of TSH and LH/CG Receptor cDNAs from Human Thyroid: Regulation by Tissue Specific Splicing, Molecular Endocrinology, Volume 4, Issue 8, 1 August 1990, Pages 1264–1276, https://doi.org/10.1210/mend-4-8-1264
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.
This content is only available as a PDF.
Author notes
This work was supported by NIH Grants DK-41921-01 and the Medical Research Foundation (to R.D.C.) and by NIH Grant HD-22196(to D.L.S.).
A.L.F. was supported by a fellowship from the Association for Brain Tumor Research.
*
Present address: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland Oregon 97201; to whom requests for reprints should be addressed.
Copyright © 1990 by The Endocrine Society
Citations
Views
Altmetric
Metrics
Total Views 393
26 Pageviews
367 PDF Downloads
Since 5/1/2017
| Month: | Total Views: |
|---|---|
| May 2017 | 2 |
| July 2017 | 2 |
| August 2017 | 6 |
| September 2017 | 2 |
| October 2017 | 2 |
| December 2017 | 4 |
| January 2018 | 6 |
| February 2018 | 12 |
| March 2018 | 9 |
| April 2018 | 9 |
| May 2018 | 3 |
| June 2018 | 2 |
| July 2018 | 12 |
| August 2018 | 34 |
| September 2018 | 7 |
| October 2018 | 8 |
| November 2018 | 5 |
| December 2018 | 10 |
| February 2019 | 2 |
| March 2019 | 5 |
| April 2019 | 12 |
| May 2019 | 1 |
| June 2019 | 3 |
| July 2019 | 5 |
| August 2019 | 9 |
| September 2019 | 6 |
| October 2019 | 16 |
| November 2019 | 3 |
| December 2019 | 3 |
| January 2020 | 12 |
| February 2020 | 8 |
| March 2020 | 19 |
| April 2020 | 3 |
| May 2020 | 1 |
| June 2020 | 5 |
| July 2020 | 7 |
| August 2020 | 12 |
| September 2020 | 3 |
| October 2020 | 3 |
| December 2020 | 3 |
| January 2021 | 7 |
| February 2021 | 5 |
| March 2021 | 3 |
| April 2021 | 3 |
| May 2021 | 1 |
| June 2021 | 4 |
| August 2021 | 1 |
| September 2021 | 7 |
| October 2021 | 1 |
| November 2021 | 4 |
| December 2021 | 2 |
| January 2022 | 2 |
| February 2022 | 2 |
| April 2022 | 4 |
| May 2022 | 2 |
| June 2022 | 1 |
| July 2022 | 1 |
| August 2022 | 1 |
| October 2022 | 1 |
| November 2022 | 2 |
| December 2022 | 3 |
| January 2023 | 1 |
| February 2023 | 3 |
| March 2023 | 1 |
| April 2023 | 1 |
| May 2023 | 3 |
| June 2023 | 1 |
| July 2023 | 2 |
| August 2023 | 1 |
| September 2023 | 2 |
| October 2023 | 4 |
| November 2023 | 1 |
| December 2023 | 1 |
| January 2024 | 1 |
| February 2024 | 8 |
| March 2024 | 7 |
| April 2024 | 4 |
| May 2024 | 4 |
| June 2024 | 5 |
| July 2024 | 5 |
| August 2024 | 3 |
| September 2024 | 1 |
| October 2024 | 1 |
Citations
160 Web of Science
×
Email alerts
Related articles in PubMed
Citing articles via
More from Oxford Academic