Regulation and activation of focal adhesion kinase and paxillin during the adhesion, proliferation, and differentiation of prostatic epithelial cells in vitro and in vivo. (original) (raw)
Journal Article
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
,
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
1Department of Surgery (Urology), McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
Search for other works by this author on:
Published:
01 August 1996
PDF Cite
L Tremblay, W Hauck, L T Nguyen, P Allard, F Landry, A Chapdelaine, S Chevalier, Regulation and activation of focal adhesion kinase and paxillin during the adhesion, proliferation, and differentiation of prostatic epithelial cells in vitro and in vivo., Molecular Endocrinology, Volume 10, Issue 8, 1 August 1996, Pages 1010–1020, https://doi.org/10.1210/mend.10.8.8843417
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Focal adhesion kinase (pp125FAK) is a nonreceptor protein tyrosine kinase transducing signals initiated through integrin activation triggered by cell/extracellular matrix (ECM) interactions. To examine its role in epithelial cell adhesion, proliferation, and differentiation, we have studied pp125FAK expression, activity, and association with paxillin in two canine prostate models in which these functions can be selectively regulated: in vitro by vitronectin (VN) and serum factors, and in vivo by sex steroids. Kinetic studies revealed that the adhesion and spreading of prostatic epithelial cells in primary culture was regulated by serum VN and a natural ECM containing VN produced by prostate cells. While barely detectable in freshly isolated prostate cells, proliferating cells, after 72 h in culture, expressed higher levels of FAK mRNA (8-fold), pp125FAK (50-fold), and paxillin (50-fold). In prostate cells with a reduced growth rate after 2 weeks in culture, we observed a decrease in pp125FAK (4-fold) and its transcript (3-fold), but no change in paxillin. In vivo, both proteins were undetectable in normal and hyperplastic glands composed of a well differentiated epithelium, and in prostates restored by androgen supplementation. In contrast, pp125FAK and paxillin were up-regulated by androgen deprivation (castration) and further increased by estrogen treatment, which yielded metaplastic prostates mostly composed of proliferating basal epithelial cells. Moreover, both proteins were constitutively phosphorylated on tyrosine in the metaplastic prostate, as well as in proliferating cultured cells. Together, these results demonstrate that pp125FAK expression is regulated at the protein and mRNA levels and forms active signaling complexes with paxillin when epithelial cells in contact with ECM proteins are induced to proliferate in vivo and in vitro.
This content is only available as a PDF.
Copyright © 1996 by The Endocrine Society
Citations
Views
Altmetric
Metrics
Total Views 314
16 Pageviews
298 PDF Downloads
Since 1/1/2017
| Month: | Total Views: |
|---|---|
| January 2017 | 1 |
| May 2017 | 1 |
| June 2017 | 1 |
| August 2017 | 2 |
| September 2017 | 1 |
| October 2017 | 2 |
| November 2017 | 1 |
| December 2017 | 8 |
| January 2018 | 4 |
| February 2018 | 3 |
| March 2018 | 3 |
| April 2018 | 3 |
| May 2018 | 2 |
| June 2018 | 7 |
| July 2018 | 10 |
| August 2018 | 3 |
| September 2018 | 3 |
| October 2018 | 4 |
| November 2018 | 1 |
| December 2018 | 4 |
| January 2019 | 4 |
| February 2019 | 3 |
| March 2019 | 8 |
| April 2019 | 8 |
| May 2019 | 6 |
| June 2019 | 8 |
| July 2019 | 6 |
| August 2019 | 5 |
| September 2019 | 4 |
| October 2019 | 8 |
| November 2019 | 5 |
| December 2019 | 6 |
| January 2020 | 10 |
| February 2020 | 8 |
| March 2020 | 12 |
| April 2020 | 5 |
| May 2020 | 4 |
| June 2020 | 6 |
| July 2020 | 7 |
| August 2020 | 10 |
| September 2020 | 3 |
| October 2020 | 12 |
| November 2020 | 5 |
| December 2020 | 2 |
| January 2021 | 1 |
| February 2021 | 1 |
| March 2021 | 4 |
| April 2021 | 3 |
| May 2021 | 3 |
| June 2021 | 1 |
| July 2021 | 1 |
| September 2021 | 3 |
| October 2021 | 2 |
| November 2021 | 1 |
| December 2021 | 2 |
| February 2022 | 2 |
| March 2022 | 4 |
| April 2022 | 4 |
| July 2022 | 2 |
| August 2022 | 2 |
| September 2022 | 2 |
| October 2022 | 6 |
| December 2022 | 1 |
| February 2023 | 1 |
| March 2023 | 1 |
| June 2023 | 1 |
| July 2023 | 3 |
| August 2023 | 4 |
| September 2023 | 3 |
| October 2023 | 4 |
| November 2023 | 5 |
| December 2023 | 1 |
| January 2024 | 1 |
| February 2024 | 5 |
| March 2024 | 1 |
| April 2024 | 2 |
| May 2024 | 2 |
| June 2024 | 2 |
| July 2024 | 3 |
| August 2024 | 7 |
| September 2024 | 3 |
| October 2024 | 1 |
×
Email alerts
Related articles in PubMed
Citing articles via
More from Oxford Academic