Telophase disc: a new mammalian mitotic organelle that bisects telophase cells with a possible function in cytokinesis (original) (raw)
RESEARCH ARTICLE| 01 July 1991
1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
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1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
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3
Departments of Laboratory Medicine and Medicine
,
University of ‘Washington
,
Seattle, WA 98195, USA
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1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
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Paul R. Andreassen
1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
Douglas K. Palmer
1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
Mark H. Wener
3
Departments of Laboratory Medicine and Medicine
,
University of ‘Washington
,
Seattle, WA 98195, USA
Robert L. Margolis
1
The Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA 98104, USA
Online ISSN: 1477-9137
Print ISSN: 0021-9533
© 1991 by Company of Biologists
1991
J Cell Sci (1991) 99 (3): 523–534.
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ABSTRACT
We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, encompasses the entire midzone diameter, and its distribution survives disassembly of interpolar microtubules by cold temperature treatment and detergent lysis of cells. Cytokinetic furrow contraction proceeds normally in dihydrocytochalasin B (DCB)-treated cells, and antigen distribution in the furrow is unaltered. In DCB, the furrow retracts in early interphase, coincident with loss of normal membrane association with the disc, resulting in the formation of binucleate cells. The midzone disc in both drug-treated and normal cells is present at the correct time and position to play a central role in cytokinesis. By immunocytochemistry, the disc appears to contain myosin but not actin. The position of the disc and the possible presence of myosin suggest that cytokinesis may involve the interaction of the disc organelle with actin in the cell cortex to produce cleavage in mammalian cells.
© 1991 by Company of Biologists
1991
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