Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells (original) (raw)

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Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

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Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

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,

Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

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,

Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

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,

Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

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Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

Department of Applied and Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

, 3–5–8 Saiwaicho, Fuchu, Tokyo 183,

Japan

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Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

, 2–24–16 Nakamachi, Koganei, Tokyo 184,

Japan

Department of Applied and Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

, 3–5–8 Saiwaicho, Fuchu, Tokyo 183,

Japan

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Published:

01 January 1996

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Kazuaki Yoshioka, Mikako Saito, Ki-Bong Oh, Yasuyuki Nemoto, Hideaki Matsuoka, Masahiro Natsume, Hiroshi Abe, Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in Escherichia coli Cells, Bioscience, Biotechnology, and Biochemistry, Volume 60, Issue 11, 1 January 1996, Pages 1899–1901, https://doi.org/10.1271/bbb.60.1899
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Abstract

A fluorescent derivative of D-glucose, 2-NBDG, which was previously developed for the evaluation of glucose uptake activity by living cells, was used on Escherichia coli cells and its fate after incorporation in the cells was investigated. 2-NBDG was converted to another fluorescent derivative (2-NBDG metabolite) immediately after it was taken by E. coli cells. This 2-NBDG metabolite was then decomposed to non-fluorescent forms. 2-NBDG metabolite was decomposed into the original 2-NBDG by G6Pase with concurrent liberation of inorganic phosphate. Furthermore, FAB/MS analysis showed that its molecular weight was 420, the same value as that of 2-NBDG 6-phosphate. These indicate 2-NBDG metabolite should be 2-NBDG 6-phosphate. Based on these results, the feasibility of 2-NBDG as a fluorescent non-toxic probe for glucose uptake activity and its application to viability assessment of various living systems are discussed.

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© 1996 Taylor and Francis Group LLC

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