Rapid Detection and Quantitation of BRAF Mutations in Hairy Cell Leukemia Using a Sensitive Pyrosequencing Assay (original) (raw)

Journal Article

,

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

2Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

Carlos E. Bueso-Ramos, MD, PhD

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

2Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

2Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

2Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

,

1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX

Search for other works by this author on:

... Show more

Published:

07 January 2012

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Shalini Verma, Wesley O. Greaves, Farhad Ravandi, Neelima Reddy, Carlos E. Bueso-Ramos, Susan O’Brien, Deborah A. Thomas, Hagop Kantarjian, L. Jeffrey Medeiros, Rajyalakshmi Luthra, Keyur P. Patel, Rapid Detection and Quantitation of BRAF Mutations in Hairy Cell Leukemia Using a Sensitive Pyrosequencing Assay, American Journal of Clinical Pathology, Volume 138, Issue 1, July 2012, Pages 153–156, https://doi.org/10.1309/AJCPL0OPXI9LZITV
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

BRAF protooncogene is an important mediator of cell proliferation and survival signals. BRAF p.V600E mutation was recently described as a molecular marker of hairy cell leukemia (HCL). We developed and validated a pyrosequencing-based approach that covers BRAF mutational hotspots in exons 11 (codon 468) and 15 (codons 595 to 600). The assay detects BRAF mutations at an analytical sensitivity of 5%. We screened 16 unenriched archived bone marrow aspirate samples from patients with a diagnosis of HCL (n = 12) and hairy cell leukemia–variant (HCL-v) (n = 4) using pyrosequencing. BRAF p.V600E mutation was present in all HCL cases and absent in all HCL-v. Our data support the recent finding that BRAF p.V600E mutation is universally present in HCL. Moreover, our pyrosequencing-based assay provides a convenient, rapid, sensitive, and quantitative tool for the detection of BRAF p.V600E mutations in HCL for clinical diagnostic testing.

© American Society for Clinical Pathology

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Rapid Detection and Quantitation of BRAF Mutations in Hairy Cell Leukemia Using a Sensitive Pyrosequencing Assay - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 1,122

726 Pageviews

396 PDF Downloads

Since 12/1/2016

Citations

34 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic