Superresolution by image scanning microscopy using pixel reassignment (original) (raw)
- Optics Letters
- Vol. 38,
- Issue 15,
- pp. 2889-2892
- (2013)
- •https://doi.org/10.1364/OL.38.002889
Author Information
Author Affiliations
Colin J. R. Sheppard,1,*Shalin B. Mehta,2and Rainer Heintzmann3,4,5
1Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
2Marine Biological Laboratory, Woods Hole, Massachusetts 02543, USA
3Institute of Photonic Technology, Albert Einstein Str. 9, 07745 Jena, Germany
4Institute of Physical Chemistry, Abbe Centre of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 5, 07743 Jena, Germany
5King’s College London, Randall Division of Cell and Molecular Biophysics, SE1 1UL London, UK
*Corresponding author: colinjrsheppard@gmail.com
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Abstract
The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.
© 2013 Optical Society of America
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