Distinct Mechanisms Determine Transposon Inheritance and Methylation via Small Interfering RNA and Histone Modification (original) (raw)
Figure 3
Southern Blot Analysis
(A and B) Genomic DNAs prepared from 4-wk-old plants of the indicated mutant and backcrossed (m/+) genotypes were digested with either HindIII and HpaII (left) or HindIII and MspI (right) and used for Southern blot analysis with a probe specific to the DNA transposons AtMu1 and the retrotransposon ATCOPIA4. The L_er_ genotype is shown. DNA methylation loss for each element within the mutants and their backcrosses is indicated by loss of band intensity relative to WT as indicated by the arrows or brackets.
(C) Genomic DNAs from the same genotypes in (A) and (B) were digested with either HpaII (left) or MspI (right) and used for Southern blot analysis with a probe specific to the ATLINE1-4 element. The probe corresponds to a region flanked on both sides by more than five HpaII/MspI sites within 6 kb. Thus, fragment sizes generated upon digestion of the genotypes tested varied owing to a number of potential methylation changes. The fragments within the brackets depict significant changes in methylation between the genotypes.