Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (original) (raw)
Figure 5
The Manual Annotation Flow Chart of ncRNAs
Candidate non-protein-coding genes were compared with the human genome, ESTs, cDNA 3′-end features and the locus genomic environment. The candidates were then classified into four categories: hold (cDNAs improperly mapped onto the human genome); uncharacterized transcripts (transcripts overlapping a sense gene or located within 5 kb of a neighboring gene with EST support); putative ncRNAs (multiexon or single exon transcripts supported by ESTs or 3′-end features); and unclassifiable (possible genomic fragments).