Hairy Transcriptional Repression Targets and Cofactor Recruitment in Drosophila (original) (raw)
Figure 3
hairy Affects stg-lacZ Reporter Expression
(A–F) β-galactosidase expression from the stg-lacZ reporter lines pstg β-E4.9 (A and B), pstg β-E6.4 (C and D), and pstg β-E6.7 (E and F) in wild-type (A, C, and E) and hairy mutant (B, D, and F) embryos. Note the expanded (de-repressed) lacZ expression in the hairy mutant background compared to wild-type for the E4.9 and E6.4 lines (compare [B] to [A] and [D] to [C], respectively).
(G) β-galactosidase expression from the stg-lacZ reporter line pstg β-E4.9ΔHairy (same as the reporter construct shown in [A], but with a Hairy binding site mutation) in a wild-type background. Note the expanded (de-repressed) lacZ expression (compare with [A]). Anterior is to the left. Dorsal is up in (A–D) and (G), whereas the ventral surface is shown in (E–F).