Hairy Transcriptional Repression Targets and Cofactor Recruitment in Drosophila (original) (raw)
Figure 6
Hairy Binds to Putative Target Loci on Polytene Chromosomes
(A) Hairy binds to polytene region 1A, the location of the Hairy target, ac.
(B) Hairy is not found at 84A, the cytological location for ftz.
(C–F) Hairy also binds to polytene region 99A, the location of stg (C); polytene region 3A, the location of egh (D); polytene region 33C, the location of prd (E); and polytene region 82A, the location of hkb (F).
(G–I) Hairy is recruited to the insertion site for the pstg βE-4.9 reporter construct (arrow in [H] and [I]). Compare to the equivalent region of wild-type X chromosomes marked by brackets in (A), (D), and (G).
(J) In situ hybridization to polytene chromosomes from pstg βE-4.9 larvae showing that this line has two insertions on the X chromosome at 1F and 6C. The probe also recognizes sequences to the endogenous white locus (asterisk).