Two Distinct E3 Ubiquitin Ligases Have Complementary Functions in the Regulation of Delta and Serrate Signaling in Drosophila (original) (raw)
Figure 3
D-mib Co-Localizes with Dl and Ser at the Apical Cell Cortex
(A and A′) D-mib (green) is detected in all cells of the wing imaginal disc. In (A), Ser is in red and Discs-large (Dlg) is in blue.
(B–D′′′) D-mib (green in B, B′, C, C′, D, and D′) co-localized with Ser (red in [B and B′′]), Dl (red in [C and C′′]), N (red in [D and D′′]), and E-Cadherin (E-Cad; blue in [D and D′′′]) and was found apical to Discs-large (Dlg; blue in [B, B′′′, C, and C′′′]) in notum cells located at the edges of the wing discs.
(E–E′′) D-mib (green in [E and E′]) co-localized with Dl (red in [E and E′′]) at the apical cortex of wing pouch cells.
(F–F′′) D-mib staining at the apical cortex (blue in [F and F′]) was not detected in D-mib2 mutant clone (marked by loss of nuclear GFP staining; green in [F]). Loss of D-mib activity has no detectable effect on the apical accumulation of Dl (red in [F and F′′]).
Bar is 50 μm for (A and A′) and 10 μm for (B–F′′).