Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (original) (raw)
Figure 1
Generation of NS Cells from ES Cells
(A) The adherent NS cell culture (LC1) propagated in EGF and FGF-2 (a), shows no expression of neuronal (b) or astrocyte (c) antigens, and uniform expression of the precursor marker RC2 (d) and nestin (not shown). LC1 cells differentiate into GFAP immunopositive astrocytes (e) upon addition of serum and generate TuJ1 immunopositive neurons (f) upon growth factor withdrawal.
(B) The proportion of neurons obtained remains greater than 35% of total cells after 115 passages (g). Immunostaining for MAP2 of LC1 differentiation shown at passage 16 (h) and passage 158 (i).