Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (original) (raw)
Figure 2
Clonal NS Cells Generated through Sox1 Neural Lineage Selection
(A) Phase image of neural precursors at passage 1 (a) and 5 (c), with (b) and (d) showing corresponding _Sox1_-GFP fluorescence. Image (e) shows a single cell, 1 h after plating in Terasaki well, and (f) shows a phase-contrast image of clonal cell line at passage 20.
(B) Differentiation of NS-5 cells into astrocytes (g,h) and neurons (j,k) with loss of nestin immunoreactivity (i,l).
(C) These NS-5 cells are immunoreactive for neural precursor cell/radial glia markers (m–o,q,r) and negative for GFAP (p).
(D) Clones of NS-5 cells exhibit homogenous expression of BLBP with no immunoreactivity for GFAP in the presence of EGF/FGF (s), and generate neurons upon growth factor withdrawal (t).
(E) Metaphase spread of NS-5 (passage 31).