Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (original) (raw)
Figure 3
NS Cells Die or Begin to Differentiate in the Absence of EGF
Unlike proliferating cultures in FGF plus EGF (A,C), NS cells on gelatin die by caspase-3-mediated programmed cell death 20 h after removal of EGF (B,D). This death can be overcome if cells are cultured on a laminin substrate in FGF-2 only (F). Under these conditions, cells become slow-dividing and extend longer processes (G,H). Most cells retain RC2 immunoreactivity (H), but a minority begin neuronal differentiation marked by TuJ1 expression (J).