Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (original) (raw)
Figure 6
NS Cells Incorporate and Differentiate within the Adult Brain
(A–H) Confocal images of LC1 NS cells, lentivirally transduced with enhanced GFP, 4 wk post-grafting into hippocampus (A,B) or striatum (C–H). (B) and (D) show higher magnification of the insets in panels (A) and (C), respectively. Examples of enhanced GFP grafted NS cells (green) showing co-expression (yellow) of the neuronal markers TuJ (E, red) or MAP-2 (F, red), astroglial marker GFAP (G, red), neural progenitor marker nestin (H, red).
(I) Quantitative analysis of graft-derived neuronal (MAP2), astroglial (GFAP), progenitor (Nestin), and proliferating (Ki67) cells, 4 wk after transplantation into adult mouse striatum. Data are means (± standard deviation) of at least 500 enhanced GFP+ cells from five independent animals. DG, Dentate Gyrus; ST, Striatum. Scale bars: A,C, 100 μm; B, D, E, 40 μm.; F–H, 20 μm.