Impaired DNA Replication within Progenitor Cell Pools Promotes Leukemogenesis (original) (raw)
Figure 6
HU Selects for Bcr-Abl Expression Within Hematopoietic Progenitor Pools In Vivo
BALB/c c-Kit+ BM cells were transduced with the indicated retroviruses, and transplanted into sublethally irradiated BALB/c recipients. At 2 wk post-BMT, half of each group was switched to water containing HU.
(A) BM cells were analyzed for the expression of Lin antigens, Sca1, and GFP as described in Figure 2C on days 5 and 8 of HU treatment. Cells analyzed in the profiles shown were gated as Lin−. The percentages of the progenitor enriched Lin− Sca1+ population that expressed vector or Bcr-Abl are indicated in the upper right corners (underlined).
(B) Four days after switching to HU water (or not), mice were injected with BrdU, and spleen GFP+ and GFP− Lin− progenitors isolated by flow sorting as in Figure 3. BrdU incorporation is detected in GFP− cells (internal control) and GFP+ cells, and the calculated average lengths of S phase from two experiments is shown in the graph.