Surviving Endoplasmic Reticulum Stress Is Coupled to Altered Chondrocyte Differentiation and Function (original) (raw)
Figure 6
The Signal for the Altered Differentiation of 13del HCs Is Cell Autonomous
Analyses of EGFP/wt and EGFP/13del chimeras.
(A, B, and C) In EGFP/13del chimeras, normal (wt) cells express EGFP.
(D, E, and F) 13del cells are identified by in situ hybridization with a 64-bp probe specific for the 13del transcripts. (D′) shows expression of Col10a1 in the normal EGFP/wt chimera (consecutive section).
(G–I) Immunohistochemical detection for BiP.
(J–L) Immunohistochemical detection for p57Kip2.
(M–O) In situ hybridization for Ppr. The insets in (M) and (N) show regions in the LHZ containing wt and 13del cells, respectively, at higher magnification. (N) shows marked re-expression of Ppr in 13del cells.
Chondro-osseous junctions are traced using a red or yellow line. Color contrast of (A–C) and (G–L) was adjusted as described in Materials and Methods. Bar indicates 100 μm.