Surviving Endoplasmic Reticulum Stress Is Coupled to Altered Chondrocyte Differentiation and Function (original) (raw)
Figure 9
Correlation of Altered Differentiation of HCs and ERSS
Serial transverse sections of 10-d-old tibiae were collected for quantitative RT-PCR analyses. A section between each fraction of 20 sections was stained with safranin-O and the von Kossa stain to identify the bony or cartilaginous nature of each fraction. The section around the chondro-osseous junction is shown from wt (A) and 13del (B) mice. Mineralized tissues stained dark brown and cartilaginous tissues stained orange. Relative transcript abundance was quantified for Col10a1 and 13del, BiP, and Mmp9 in wt (A) and 13del (B). The Col10a1 primers also recognize 13del transcripts. Fractions are numbered from the bone shaft towards the growth plate cartilage for reference only, and there is no correlation between wt and 13del fractions. The position of the fractions relative to the bone and HZ of the growth plate is indicated. The HZ is delineated by a pair of dotted line in each graph; PZ and RZ are on the right-hand side of the HZ. Error bars represent ±1 standard deviation (SD).