LACHESIS Restricts Gametic Cell Fate in the Female Gametophyte of Arabidopsis (original) (raw)
Figure 4
Successive Recruitment of Cells as Gametic Cells in lis-1 Gametophytes
(A and B) Ectopic expression of the egg cell marker ET1119 (A) and the central cell marker pMEA::GUS (B) in wild-type and lis-1/LIS plants. Dark bars represent wild-type, light bars represent lis-1/LIS plants. The _y_-axis shows the percentage of ectopic expression of total GUS-staining ovules. Ectopic expression was scored zero (0d), one (1d), and two days (2d) after emasculation. Total number of ovules counted was greater than 250.
(C–G) Development of five morphological features in lis-1/LIS plants as compared to wild type, zero (0d), one (1d), and two days (2d) after emasculation. The _y_-axis shows percent deviation from wild type (data from Table S1). (C) Synergid nuclei smaller than egg cell nucleus. (D) Different polarity of synergids and egg cell with respect to position of nucleus. (E) Polar nuclei unfused. (F) Ectopic cellularization. (G) Protruded antipodal cells.