Protein Oxidation Implicated as the Primary Determinant of Bacterial Radioresistance (original) (raw)
Figure 3
In Vivo IR-Induced Oxidative Protein Damage
Samples were treated (+) or not treated (−) with DNPH. For the Western blot (W) and for the Coomassie-stained polyacrylamide denaturing gel (C), 20-μg protein samples were loaded per lane. DR/Fe/0.4 kGy indicates that the D. radiodurans (non-irradiated) cell extract was adjusted to 500 μM FeCl2 and exposed to 0.4 kGy in vitro. Values for intracellular Mn/Fe concentration ratios and D10 at the bottom of the figure, as reported previously [1]. M, mixture of artificial IgG-binding protein standards; O, oxidized protein standards; S, wide-range protein standards; SO, S. oneidensis.