Protein Oxidation Implicated as the Primary Determinant of Bacterial Radioresistance (original) (raw)
Figure 5
Mn-Dependent IR-Driven O2 and H2O2 Generation
(A) Column I. No IR, aerobic; II. No IR, anaerobic (pre-conditioned by purging with Ar [ultra-high purity]); III. 10 kGy, anaerobic; IV. 10 kGy, anaerobic, followed by incubation at 32 °C for 60 min; V. As for column III, but re-purged with Ar after irradiation; and VI. As for column III, but treated with catalase (15,000 units) after irradiation and then re-purged with Ar. O2/H2O2 concentration determined by the Rhodazine D assay. See Figure S2 for additional assays. Irradiations (60Co) were at 0 °C. MnCl2 solutions and bacteria (1.6 × 109 cells/ml) were prepared in dH2O (pH ∼6).
(B) Standards.