Peptides Encoded by Short ORFs Control Development and Define a New Eukaryotic Gene Family (original) (raw)
Figure 3
tal Has a Morphogenetic Function
Optical sections of the third instar leg imaginal discs. The discs are shown in a side view with dorsal up and distal to the right, and the tissue morphology is revealed by phalloidin-rhodamine (red) staining of the actin cytoskeleton and anti-β-integrin (green, yellow overlap) staining of basal membranes. The position of the tarsal fold (ventral side) is indicated with an arrowhead.
(A–A'') Morphological changes in a wild-type leg disc. At 84 h, the tarsal fold starts to form as an apico-basal constriction of the epithelial cells. At 96 h, this constriction is followed by invagination of the cells. At 110 h, cells that originated in the tarsal fold form secondary folds that constitute the primordia of the tarsal segments.
(B–B'') In a tal1 mutant, the original tarsal constriction forms as in the wild type, but the tarsal fold never forms, and basal integrin staining remains stronger than in the wild type.