Higher-Order Oligomerization Targets Plasma Membrane Proteins and HIV Gag to Exosomes (original) (raw)
Figure 9
Vesicular Secretion of a Synthetic Exosomal Cargo
(A–H) Fluorescence micrographs of N-F-PE–labeled Jurkat T cells expressing (A–D) Acyl-LZ-DsREDmonomer or (E–H) Acyl-LZ-DsRED.
(I–L) Fluorescence micrographs of exosomes secreted by N-F-PE–labeled Jurkat T cells expressing (I and J) Acyl-LZ-DsREDmonomer or (K and L) Acyl-LZ-DsRED. White circles mark exosomes that contain both N-F-PE and Acyl-LZ-DsRED. Bar indicates 10 μm.
(M) Anti-DsRED immunoblot of exosome and cell lysates of Jurkat T cells expressing (left lanes) Acyl-LZ-DsREDmonomer or (right lanes) Acyl-LZ-DsRED (equal ratios of exosome lysate:cell lysate).