Aging Hematopoietic Stem Cells Decline in Function and Exhibit Epigenetic Dysregulation (original) (raw)
Figure 4
Up-Regulation of P-selectin Cell Surface Expression and NF-κB Localization in Aged HSCs.
(A) An increasing percentage of HSCs express P-selectin (SelP) when examined by FACS, ranging from 3% (2-mo-old HSCs) to 81% (28-mo-old HSCs). FITC, fluorescein isothiocyanate; PE, phycoerythrin.
(B and C) HSCs stained with anti-p65 NF-kB antibody (red) and DAPI (blue). Two-month-old HSCs contain approximately 3% nuclear-localized p65; however, at 22 mo, approximately 71% show nuclear-localized p65.