Characterization of Sleep in Zebrafish and Insomnia in Hypocretin Receptor Mutants (original) (raw)
Figure 5
hcrtr Coexpression with GABAergic, Adrenergic, Cholinergic, and Glycinergic Systems in 2-dpf Larvae
Results were all obtained using ISH.
(A) Lateral view of a flat-mounted hemi-brain stained for hcrtr (blue) and gad67 (red). Note multiple areas of overlap indicated by black arrowheads.
(B) Lateral view of a flat-mounted hemi-brain stained for hcrtr (blue) and adra2a (red). Double staining in the anterior hypothalamus and telencephalon is indicated by arrowheads.
(C) Lateral view of a flat-mounted hemi-brain stained for hcrtr (blue) and chat (red). Double-stained regions are indicated by black arrowheads.
(D) Lateral views of spinal cord stained for hcrtr (blue) and glyt2 (red). Note that most hcrtr neurons but not all (white arrowhead) are glycinergic.
(E) Lateral views of spinal cord stained for hcrtr (blue) and gad67 (red). Note that all hcrtr neurons are GABAergic.