Distinct Roles for Arp2/3 Regulators in Actin Assembly and Endocytosis (original) (raw)
Figure 1
Acidic Domains of Arp2/3 Activators, Model of Actin Patch Assembly and MSD Examples
(A) Alignment of acidic/DDW regions of S. cerevisiae Arp2/3 activators with the corresponding regions of human WASp and N-WASp. Acidic residues are highlighted with gray and the conserved tryptophan with black. Numbers are amino acid residues. The entire region shown here was removed in “Δ_acidic_” strains.
(B) Model of actin patch formation and movement, adapted from previous studies [23,24,33], are shown.
(C) Examples of squared displacement plotted versus time for five individual patches, with the curves aligned at the start (left) or (right) end of patch lifetime. Such curves would then be averaged to generate MSD plots.
(D) Typical MSD plots of wild-type patches labeled with Sla2-GFP generated by aligning individual curves at the start (left) or the end (right) of patch lifetime. On the left, the plot is truncated when half of the patches have disappeared, indicating the median lifetime. Phases I and II of patch movement are indicated.
(E) Typical MSD plots of wild-type patches labeled with Abp1-GFP generated as in (D). Phase III patch movements are indicated.