Group II Intron Protein Localization and Insertion Sites Are Affected by Polyphosphate (original) (raw)
Figure 2
Fluorescence Microscopy of GFP/LtrA in Wild-Type E. coli and Disruptants with Altered GFP/LtrA Localization Patterns
Wild-type HMS174(DE3) (WT) and disruptants containing pACD2X-GFP/LtrA were induced with 500 μM IPTG overnight at 30 °C. For each strain, ≥200 cells were examined by fluorescence microscopy, as described in the Materials and Methods section, and the proportions of cells with different LtrA localization patterns are summarized in Table 1, top. The localization patterns shown in the figure represent the most common pattern for each strain. Bar = 2 μm.
(A) Wild-type cells showing bipolar GFP/LtrA localization and disruptants showing diffuse GFP/LtrA localization patterns.
(B) Filamentous gppA and wcaK cells showing multiple GFP/LtrA foci, diffuse patches, or a combination of the two.