A Genome-Wide RNAi Screen to Dissect Centriole Duplication and Centrosome Maturation in Drosophila (original) (raw)
Figure 3
Genes Involved in Both Centriole Duplication and PCM Recruitment (Class II)
(A) S2R+ cells treated with dsRNA against GFP (control), DCP110, and Rcd4 (CG17295) were stained with Hoechst (DNA, blue), DSas-4 (a centriole marker, red), and Cnn (a PCM marker, green). Inset shows a 4× magnified view.
(B) Recruitment of DSpd-2 (green) and γ-tubulin (red) after dsRNA treatment for control, DCP110, and Rcd4. DNA is shown in blue, and inset shows a 4× magnified view.
(C and D) Analysis of centriole (C) and centrosome (D) numbers in mitotic cells after RNAi treatment. More than 30 mitotic cells were counted in three independent experiments.
(E) Analysis of PCM size in mitotic cells after RNAi treatment. The graph represents the mean intensity of PCM staining (Cnn) from three independent experiments, each analysing more than 20 centrosomes. Error bars represent the SE; an asterisk (*) indicates p ≤ 0.05 compared to control. Note how the number of centrioles and centrosomes per cell is reduced (C and D), and the amount of PCM recruited to the remaining centrioles is also reduced (E) after DCP110 and Rcd4 depletion.
Scale bar in (A and B) represents 5 μm.